sirt fluorogenic assay kit Search Results


sirt1 (sirtuin1) fluorogenic assay kit  (AMS Biotechnology)
94
AMS Biotechnology sirt1 (sirtuin1) fluorogenic assay kit
Sirt1 (Sirtuin1) Fluorogenic Assay Kit, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sirt+fluorogenic+assay+kit/SIRT1+(Sirtuin1)+Fluorogenic+Assay+Kit/custom%4050081%4037378329
Average 94 stars, based on 1 article reviews
sirt1 (sirtuin1) fluorogenic assay kit - by Bioz Stars, 2026-10
94/100 stars
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fluorogenic assay kit  (BPS Bioscience)
94
BPS Bioscience fluorogenic assay kit
Fluorogenic Assay Kit, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sirt+fluorogenic+assay+kit/Sirtuin+6+Fluorogenic+Assay+Kit/10__3390_slash_biomedicines13102395-104-7-19
Average 94 stars, based on 1 article reviews
fluorogenic assay kit - by Bioz Stars, 2026-10
94/100 stars
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sirt3 fluorimetric activity assay kit  (BPS Bioscience)
93
BPS Bioscience sirt3 fluorimetric activity assay kit
Melatonin alleviated PM 2.5 ‐induced cardiac mitochondrial oxidative damage. A, The level of 3′‐NT. B, The level of 4‐HNE. C, The level of GSH/GSSG. D, Representative pictures of myocardial tissue by transmission electron microscope. The blow panels are high magnification (scale bar: 0.2 μm) images corresponding to the upper panels (scale bar: 0.5 μm). E, MitoSOX staining of heart tissues (scale bar: 100 μm). F, Mean fluorescence intensities of heart. G, Representative Western blot pictures. H, The quantitative analysis of <t>SIRT3</t> protein levels. I, The activity of SIRT3. J, The quantitative analysis of SOD2 acetylation. K, The activity of SOD2. Data are expressed as the means ± SD; n = 6 in each group. * P < .05
Sirt3 Fluorimetric Activity Assay Kit, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sirt+fluorogenic+assay+kit/SIRT3+(Sirtuin3)+Fluorogenic+Assay+Kit/pmc07757260-91-9-14
Average 93 stars, based on 1 article reviews
sirt3 fluorimetric activity assay kit - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier
sirt1 (sirtuin1) fluorogenic assay kit  (BPS Bioscience)
94
BPS Bioscience sirt1 (sirtuin1) fluorogenic assay kit
Melatonin alleviated PM 2.5 ‐induced cardiac mitochondrial oxidative damage. A, The level of 3′‐NT. B, The level of 4‐HNE. C, The level of GSH/GSSG. D, Representative pictures of myocardial tissue by transmission electron microscope. The blow panels are high magnification (scale bar: 0.2 μm) images corresponding to the upper panels (scale bar: 0.5 μm). E, MitoSOX staining of heart tissues (scale bar: 100 μm). F, Mean fluorescence intensities of heart. G, Representative Western blot pictures. H, The quantitative analysis of <t>SIRT3</t> protein levels. I, The activity of SIRT3. J, The quantitative analysis of SOD2 acetylation. K, The activity of SOD2. Data are expressed as the means ± SD; n = 6 in each group. * P < .05
Sirt1 (Sirtuin1) Fluorogenic Assay Kit, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sirt+fluorogenic+assay+kit/SIRT1+(Sirtuin1)+Fluorogenic+Assay+Kit/custom%4050081%40pm35175758__ja1c13555_si_001
Average 94 stars, based on 1 article reviews
sirt1 (sirtuin1) fluorogenic assay kit - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
sirt6  (BPS Bioscience)
93
BPS Bioscience sirt6
Fig. 6 SCIC2.1 indirectly activates <t>SIRT6</t> and rescues cells from metabolic stress. a Western blot analysis of SIRT6, PUMA, p21, and Bax performed in HepG2 cells treated with SCIC2.1 and Ex-527 at the indicated concentrations and time points. Heat map showing band quantification of protein expression. b SIRT6 mRNA fold expression evaluated after treatment with SCIC2.1 and Ex-527 at the indicated time points. c CETSA assay performed on HepG2 cells treated with SCIC2.1 (25 µM) and SIRT6 protein expression was analyzed. Heat map shows quantification of SIRT6 bands determined by ImageJ software. Statistical significance was calculated using the student t-test or one-way ANOVA using GraphPad Prism 9.4.0, and statistical significance is expressed as *p-value < 0.05, nsp-value > 0.05 vs control. Error bars represent the standard deviation (SD) of three biological replicates
Sirt6, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sirt+fluorogenic+assay+kit/Fluorogenic+Sirtuin+6+Substrate/pm37715252-93-9-10
Average 93 stars, based on 1 article reviews
sirt6 - by Bioz Stars, 2026-10
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cell fluorogenic f actin labeling probes sir actin  (Cytoskeleton Inc)
96
Cytoskeleton Inc cell fluorogenic f actin labeling probes sir actin
Fig. 6 SCIC2.1 indirectly activates <t>SIRT6</t> and rescues cells from metabolic stress. a Western blot analysis of SIRT6, PUMA, p21, and Bax performed in HepG2 cells treated with SCIC2.1 and Ex-527 at the indicated concentrations and time points. Heat map showing band quantification of protein expression. b SIRT6 mRNA fold expression evaluated after treatment with SCIC2.1 and Ex-527 at the indicated time points. c CETSA assay performed on HepG2 cells treated with SCIC2.1 (25 µM) and SIRT6 protein expression was analyzed. Heat map shows quantification of SIRT6 bands determined by ImageJ software. Statistical significance was calculated using the student t-test or one-way ANOVA using GraphPad Prism 9.4.0, and statistical significance is expressed as *p-value < 0.05, nsp-value > 0.05 vs control. Error bars represent the standard deviation (SD) of three biological replicates
Cell Fluorogenic F Actin Labeling Probes Sir Actin, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sirt+fluorogenic+assay+kit/SiR-Actin+Kit/pm38399695-83-29-35
Average 96 stars, based on 1 article reviews
cell fluorogenic f actin labeling probes sir actin - by Bioz Stars, 2026-10
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fluorogenic sirt5 assay kit  (BPS Bioscience)
93
BPS Bioscience fluorogenic sirt5 assay kit
Fig. 6 SCIC2.1 indirectly activates <t>SIRT6</t> and rescues cells from metabolic stress. a Western blot analysis of SIRT6, PUMA, p21, and Bax performed in HepG2 cells treated with SCIC2.1 and Ex-527 at the indicated concentrations and time points. Heat map showing band quantification of protein expression. b SIRT6 mRNA fold expression evaluated after treatment with SCIC2.1 and Ex-527 at the indicated time points. c CETSA assay performed on HepG2 cells treated with SCIC2.1 (25 µM) and SIRT6 protein expression was analyzed. Heat map shows quantification of SIRT6 bands determined by ImageJ software. Statistical significance was calculated using the student t-test or one-way ANOVA using GraphPad Prism 9.4.0, and statistical significance is expressed as *p-value < 0.05, nsp-value > 0.05 vs control. Error bars represent the standard deviation (SD) of three biological replicates
Fluorogenic Sirt5 Assay Kit, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sirt+fluorogenic+assay+kit/SIRT5+(Sirtuin5)+Fluorogenic+Assay+Kit/pm36095012-421-7-11
Average 93 stars, based on 1 article reviews
fluorogenic sirt5 assay kit - by Bioz Stars, 2026-10
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sirt3 (sirtuin3) fluorogenic assay kit  (AMS Biotechnology)
92
AMS Biotechnology sirt3 (sirtuin3) fluorogenic assay kit
Fig. 6 SCIC2.1 indirectly activates <t>SIRT6</t> and rescues cells from metabolic stress. a Western blot analysis of SIRT6, PUMA, p21, and Bax performed in HepG2 cells treated with SCIC2.1 and Ex-527 at the indicated concentrations and time points. Heat map showing band quantification of protein expression. b SIRT6 mRNA fold expression evaluated after treatment with SCIC2.1 and Ex-527 at the indicated time points. c CETSA assay performed on HepG2 cells treated with SCIC2.1 (25 µM) and SIRT6 protein expression was analyzed. Heat map shows quantification of SIRT6 bands determined by ImageJ software. Statistical significance was calculated using the student t-test or one-way ANOVA using GraphPad Prism 9.4.0, and statistical significance is expressed as *p-value < 0.05, nsp-value > 0.05 vs control. Error bars represent the standard deviation (SD) of three biological replicates
Sirt3 (Sirtuin3) Fluorogenic Assay Kit, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sirt+fluorogenic+assay+kit/SIRT3+(Sirtuin3)+Fluorogenic+Assay+Kit/custom%4050088%4010%2E1097%2Fshk%2E0000000000002366
Average 92 stars, based on 1 article reviews
sirt3 (sirtuin3) fluorogenic assay kit - by Bioz Stars, 2026-10
92/100 stars
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sirt2 assay kit  (BPS Bioscience)
93
BPS Bioscience sirt2 assay kit
Fig. 6 SCIC2.1 indirectly activates <t>SIRT6</t> and rescues cells from metabolic stress. a Western blot analysis of SIRT6, PUMA, p21, and Bax performed in HepG2 cells treated with SCIC2.1 and Ex-527 at the indicated concentrations and time points. Heat map showing band quantification of protein expression. b SIRT6 mRNA fold expression evaluated after treatment with SCIC2.1 and Ex-527 at the indicated time points. c CETSA assay performed on HepG2 cells treated with SCIC2.1 (25 µM) and SIRT6 protein expression was analyzed. Heat map shows quantification of SIRT6 bands determined by ImageJ software. Statistical significance was calculated using the student t-test or one-way ANOVA using GraphPad Prism 9.4.0, and statistical significance is expressed as *p-value < 0.05, nsp-value > 0.05 vs control. Error bars represent the standard deviation (SD) of three biological replicates
Sirt2 Assay Kit, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sirt+fluorogenic+assay+kit/SIRT2+(Sirtuin2)+Fluorogenic+Assay+Kit/pm29213065-211-5-8
Average 93 stars, based on 1 article reviews
sirt2 assay kit - by Bioz Stars, 2026-10
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fluorescent probes sir dna  (Cytoskeleton Inc)
96
Cytoskeleton Inc fluorescent probes sir dna
Fig. 6 SCIC2.1 indirectly activates <t>SIRT6</t> and rescues cells from metabolic stress. a Western blot analysis of SIRT6, PUMA, p21, and Bax performed in HepG2 cells treated with SCIC2.1 and Ex-527 at the indicated concentrations and time points. Heat map showing band quantification of protein expression. b SIRT6 mRNA fold expression evaluated after treatment with SCIC2.1 and Ex-527 at the indicated time points. c CETSA assay performed on HepG2 cells treated with SCIC2.1 (25 µM) and SIRT6 protein expression was analyzed. Heat map shows quantification of SIRT6 bands determined by ImageJ software. Statistical significance was calculated using the student t-test or one-way ANOVA using GraphPad Prism 9.4.0, and statistical significance is expressed as *p-value < 0.05, nsp-value > 0.05 vs control. Error bars represent the standard deviation (SD) of three biological replicates
Fluorescent Probes Sir Dna, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sirt+fluorogenic+assay+kit/SiR-DNA+Kit/bio_rxiv__64898__2026__05__12__724547-219-4-9
Average 96 stars, based on 1 article reviews
fluorescent probes sir dna - by Bioz Stars, 2026-10
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cell permeable reagent sir tubulin  (Cytoskeleton Inc)
96
Cytoskeleton Inc cell permeable reagent sir tubulin
Fig. 6 SCIC2.1 indirectly activates <t>SIRT6</t> and rescues cells from metabolic stress. a Western blot analysis of SIRT6, PUMA, p21, and Bax performed in HepG2 cells treated with SCIC2.1 and Ex-527 at the indicated concentrations and time points. Heat map showing band quantification of protein expression. b SIRT6 mRNA fold expression evaluated after treatment with SCIC2.1 and Ex-527 at the indicated time points. c CETSA assay performed on HepG2 cells treated with SCIC2.1 (25 µM) and SIRT6 protein expression was analyzed. Heat map shows quantification of SIRT6 bands determined by ImageJ software. Statistical significance was calculated using the student t-test or one-way ANOVA using GraphPad Prism 9.4.0, and statistical significance is expressed as *p-value < 0.05, nsp-value > 0.05 vs control. Error bars represent the standard deviation (SD) of three biological replicates
Cell Permeable Reagent Sir Tubulin, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sirt+fluorogenic+assay+kit/SiR-Tubulin+Kit/pmc06504906-139-2-5
Average 96 stars, based on 1 article reviews
cell permeable reagent sir tubulin - by Bioz Stars, 2026-10
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Image Search Results


Melatonin alleviated PM 2.5 ‐induced cardiac mitochondrial oxidative damage. A, The level of 3′‐NT. B, The level of 4‐HNE. C, The level of GSH/GSSG. D, Representative pictures of myocardial tissue by transmission electron microscope. The blow panels are high magnification (scale bar: 0.2 μm) images corresponding to the upper panels (scale bar: 0.5 μm). E, MitoSOX staining of heart tissues (scale bar: 100 μm). F, Mean fluorescence intensities of heart. G, Representative Western blot pictures. H, The quantitative analysis of SIRT3 protein levels. I, The activity of SIRT3. J, The quantitative analysis of SOD2 acetylation. K, The activity of SOD2. Data are expressed as the means ± SD; n = 6 in each group. * P < .05

Journal: Journal of Pineal Research

Article Title: Melatonin ameliorates PM 2.5 ‐induced cardiac perivascular fibrosis through regulating mitochondrial redox homeostasis

doi: 10.1111/jpi.12686

Figure Lengend Snippet: Melatonin alleviated PM 2.5 ‐induced cardiac mitochondrial oxidative damage. A, The level of 3′‐NT. B, The level of 4‐HNE. C, The level of GSH/GSSG. D, Representative pictures of myocardial tissue by transmission electron microscope. The blow panels are high magnification (scale bar: 0.2 μm) images corresponding to the upper panels (scale bar: 0.5 μm). E, MitoSOX staining of heart tissues (scale bar: 100 μm). F, Mean fluorescence intensities of heart. G, Representative Western blot pictures. H, The quantitative analysis of SIRT3 protein levels. I, The activity of SIRT3. J, The quantitative analysis of SOD2 acetylation. K, The activity of SOD2. Data are expressed as the means ± SD; n = 6 in each group. * P < .05

Article Snippet: The deacetylase activity of SIRT3 was measured by a SIRT3 Fluorimetric Activity Assay Kit (BPS bioscience) following the manufacturer's protocol.

Techniques: Transmission Assay, Microscopy, Staining, Fluorescence, Western Blot, Activity Assay

PM 2.5 regulated cardiac myofibroblast conversion via increased mitochondrial reactive oxygen levels and SOD2 acetylation, decreased SIRT3 expression and activity. A, Cardiac fibroblast cell viability after PM 2.5 ‐ treated. B, Quantification analysis of fluorescence intensity obtained from flow cytometry. C, Representative images of cardiac fibroblast α‐SMA and Vimentin immunostaining. D, Representative Western blot pictures. E, The quantitative analysis of collagen‐I, collagen‐III, and α‐SMA protein levels. F, The quantitative analysis of SIRT3 protein levels. G, The activity of SIRT3. H, The quantitative analysis of SOD2 acetylation. I, The activity of SOD2. Data are expressed as the means ± SD from three independent experiments. * P < 0.05 compared to control group

Journal: Journal of Pineal Research

Article Title: Melatonin ameliorates PM 2.5 ‐induced cardiac perivascular fibrosis through regulating mitochondrial redox homeostasis

doi: 10.1111/jpi.12686

Figure Lengend Snippet: PM 2.5 regulated cardiac myofibroblast conversion via increased mitochondrial reactive oxygen levels and SOD2 acetylation, decreased SIRT3 expression and activity. A, Cardiac fibroblast cell viability after PM 2.5 ‐ treated. B, Quantification analysis of fluorescence intensity obtained from flow cytometry. C, Representative images of cardiac fibroblast α‐SMA and Vimentin immunostaining. D, Representative Western blot pictures. E, The quantitative analysis of collagen‐I, collagen‐III, and α‐SMA protein levels. F, The quantitative analysis of SIRT3 protein levels. G, The activity of SIRT3. H, The quantitative analysis of SOD2 acetylation. I, The activity of SOD2. Data are expressed as the means ± SD from three independent experiments. * P < 0.05 compared to control group

Article Snippet: The deacetylase activity of SIRT3 was measured by a SIRT3 Fluorimetric Activity Assay Kit (BPS bioscience) following the manufacturer's protocol.

Techniques: Expressing, Activity Assay, Fluorescence, Flow Cytometry, Immunostaining, Western Blot

Mitochondrial‐derived ROS mediated PM 2.5 ‐induced cardiac myofibroblast conversion. A, Quantification analysis of fluorescence intensity obtained from flow cytometry. B, The quantitative analysis of α‐SMA protein levels. C, Representative Western blot pictures. D, The quantitative analysis of SIRT3 protein levels. E, The activity of SIRT3. F, The quantitative analysis of SOD2 acetylation. G, The activity of SOD2. Data are expressed as the means ± SD from three independent experiments. * P < .05 compared to control group; # P < .05 compared to PM 2.5 ‐treated group

Journal: Journal of Pineal Research

Article Title: Melatonin ameliorates PM 2.5 ‐induced cardiac perivascular fibrosis through regulating mitochondrial redox homeostasis

doi: 10.1111/jpi.12686

Figure Lengend Snippet: Mitochondrial‐derived ROS mediated PM 2.5 ‐induced cardiac myofibroblast conversion. A, Quantification analysis of fluorescence intensity obtained from flow cytometry. B, The quantitative analysis of α‐SMA protein levels. C, Representative Western blot pictures. D, The quantitative analysis of SIRT3 protein levels. E, The activity of SIRT3. F, The quantitative analysis of SOD2 acetylation. G, The activity of SOD2. Data are expressed as the means ± SD from three independent experiments. * P < .05 compared to control group; # P < .05 compared to PM 2.5 ‐treated group

Article Snippet: The deacetylase activity of SIRT3 was measured by a SIRT3 Fluorimetric Activity Assay Kit (BPS bioscience) following the manufacturer's protocol.

Techniques: Derivative Assay, Fluorescence, Flow Cytometry, Western Blot, Activity Assay

Melatonin alleviated the oxidative stress, SIRT3 impairment after PM 2.5 treatment in vitro. A, Cardiac fibroblast cell viability after melatonin ‐ treated. B, Quantification analysis of fluorescence intensity obtained from flow cytometry. C, The quantitative analysis of α‐SMA protein levels. D, Representative Western blot pictures. E, The quantitative analysis of SIRT3 protein levels. F, The activity of SIRT3. G, The quantitative analysis of SOD2 acetylation. H, The activity of SOD2. Data are expressed as the means ± SD from three independent experiments. * P < .05 compared to control group; # P < .05 compared to PM 2.5 ‐treated group

Journal: Journal of Pineal Research

Article Title: Melatonin ameliorates PM 2.5 ‐induced cardiac perivascular fibrosis through regulating mitochondrial redox homeostasis

doi: 10.1111/jpi.12686

Figure Lengend Snippet: Melatonin alleviated the oxidative stress, SIRT3 impairment after PM 2.5 treatment in vitro. A, Cardiac fibroblast cell viability after melatonin ‐ treated. B, Quantification analysis of fluorescence intensity obtained from flow cytometry. C, The quantitative analysis of α‐SMA protein levels. D, Representative Western blot pictures. E, The quantitative analysis of SIRT3 protein levels. F, The activity of SIRT3. G, The quantitative analysis of SOD2 acetylation. H, The activity of SOD2. Data are expressed as the means ± SD from three independent experiments. * P < .05 compared to control group; # P < .05 compared to PM 2.5 ‐treated group

Article Snippet: The deacetylase activity of SIRT3 was measured by a SIRT3 Fluorimetric Activity Assay Kit (BPS bioscience) following the manufacturer's protocol.

Techniques: In Vitro, Fluorescence, Flow Cytometry, Western Blot, Activity Assay

3‐TYP pretreatment abolished the melatonin‐suppressed cardiac myofibroblast conversion induced by PM 2.5 treatment. A, Quantification analysis of fluorescence intensity obtained from flow cytometry. B, The quantitative analysis of α‐SMA protein levels. C, Representative Western blot pictures. D, The quantitative analysis of SIRT3 protein levels. E, The activity of SIRT3. F, The quantitative analysis of SOD2 acetylation. G, The activity of SOD2. Data are expressed as the means ± SD from three independent experiments. * P < .05 compared to PM 2.5 ‐treated group; # P < .05 compared to melatonin pretreatment group; ∆ P < .05 compared to 3‐TYP pretreatment group

Journal: Journal of Pineal Research

Article Title: Melatonin ameliorates PM 2.5 ‐induced cardiac perivascular fibrosis through regulating mitochondrial redox homeostasis

doi: 10.1111/jpi.12686

Figure Lengend Snippet: 3‐TYP pretreatment abolished the melatonin‐suppressed cardiac myofibroblast conversion induced by PM 2.5 treatment. A, Quantification analysis of fluorescence intensity obtained from flow cytometry. B, The quantitative analysis of α‐SMA protein levels. C, Representative Western blot pictures. D, The quantitative analysis of SIRT3 protein levels. E, The activity of SIRT3. F, The quantitative analysis of SOD2 acetylation. G, The activity of SOD2. Data are expressed as the means ± SD from three independent experiments. * P < .05 compared to PM 2.5 ‐treated group; # P < .05 compared to melatonin pretreatment group; ∆ P < .05 compared to 3‐TYP pretreatment group

Article Snippet: The deacetylase activity of SIRT3 was measured by a SIRT3 Fluorimetric Activity Assay Kit (BPS bioscience) following the manufacturer's protocol.

Techniques: Fluorescence, Flow Cytometry, Western Blot, Activity Assay

Fig. 6 SCIC2.1 indirectly activates SIRT6 and rescues cells from metabolic stress. a Western blot analysis of SIRT6, PUMA, p21, and Bax performed in HepG2 cells treated with SCIC2.1 and Ex-527 at the indicated concentrations and time points. Heat map showing band quantification of protein expression. b SIRT6 mRNA fold expression evaluated after treatment with SCIC2.1 and Ex-527 at the indicated time points. c CETSA assay performed on HepG2 cells treated with SCIC2.1 (25 µM) and SIRT6 protein expression was analyzed. Heat map shows quantification of SIRT6 bands determined by ImageJ software. Statistical significance was calculated using the student t-test or one-way ANOVA using GraphPad Prism 9.4.0, and statistical significance is expressed as *p-value < 0.05, nsp-value > 0.05 vs control. Error bars represent the standard deviation (SD) of three biological replicates

Journal: Journal of translational medicine

Article Title: SIRT1 activation promotes energy homeostasis and reprograms liver cancer metabolism.

doi: 10.1186/s12967-023-04440-9

Figure Lengend Snippet: Fig. 6 SCIC2.1 indirectly activates SIRT6 and rescues cells from metabolic stress. a Western blot analysis of SIRT6, PUMA, p21, and Bax performed in HepG2 cells treated with SCIC2.1 and Ex-527 at the indicated concentrations and time points. Heat map showing band quantification of protein expression. b SIRT6 mRNA fold expression evaluated after treatment with SCIC2.1 and Ex-527 at the indicated time points. c CETSA assay performed on HepG2 cells treated with SCIC2.1 (25 µM) and SIRT6 protein expression was analyzed. Heat map shows quantification of SIRT6 bands determined by ImageJ software. Statistical significance was calculated using the student t-test or one-way ANOVA using GraphPad Prism 9.4.0, and statistical significance is expressed as *p-value < 0.05, nsp-value > 0.05 vs control. Error bars represent the standard deviation (SD) of three biological replicates

Article Snippet: A commercial fluorogenic assay kit for SIRT1, SIRT3, and SIRT6 (BPS Bioscience) was used to study in vitro enzymatic activity.

Techniques: Western Blot, Expressing, Software, Control, Standard Deviation